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(B ) Purity control of the sorted ICAM1-neg, ICAM1-low, and ICAM1-hi CD24-positive epithelial cell populations.
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This involved first grouping the two replicates of the control (i.e. GFP+/-) as well as that of the sorted sample's (i.e. GFP+) expression data.
After sorting, we immediately reanalyzed samples of the sorted cells.
Additional files 4 and 5 show FACS profiles of the sorted endothelial population as well as negative controls and testing of endoglin specificity.
The distributions of the sorted subpopulations became broader and shifted towards the mean of the control (compared to the original sort gates), though they remained distinctly separated (Fig. 3C, Day 0).
Side population cells ('stromal side population' cells) were separated by fluorescence-activated cell sorting from the main population of stromal cells ('stromal main population' cells) as well as from the main population tumour cells ('tumour main population' cells) (see Supplementary Fig. 4A C for gating and quality control of sorted cells).
The suffixes of the sorted elements form a sequence.
The x-axis represents the order of the sorted documents.
The present study showed that 99.4% of the sorted cells displayed green fluorescence.
The suffix array consists of the sorted suffixes of a string.
The values in each row are the original positions of the sorted source and target features.
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