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In selected experiments, a scramble peptide (SP) (YYWLQGGFAN) and nine VHCDR3 alanine-substituted derivatives were used for control of specificity and evaluation of key recognition elements of VHCDR3.
This transcomplementation strategy provides a more strict control of specificity relative to controls that involve sense, scrambled or irrelevant sequences, because it takes into account all transcript sequences present in the bacterial cell and the effector sequences remain unchanged.
Peptide competition reaction performed as control of specificity proved highly reliable detection of the HSPA2 protein in testes by our anti-HSPA2 antibody (Fig. 1d).
As a control of specificity for the immunostaining, cells that stably express the empty vector (V) yielded low reactivity after incubation with the HA antibody.
Recently, Vu et al. have identified a role for these extra loops in substrate recognition and control of specificity of reaction in the AA9 family [ 21].
As a control of specificity, amplification using primers covering regions <1 kb upstream of these sites or oligonucleotides specific for the β-actin gene resulted in non-relevant background products.
Similar(51)
23– 25 Lrp2 mRNA levels in brain extracts significantly increased along the course of the disease in GLD mice, but not EAE mice (used as controls of specificity; see Fig 4B).
Peptide block was added in parallel at a concentration of 150 ng/ml to control for specificity of the IP-reaction.
Control of stoichiometry, specificity, and reversibility allows for complex signaling systems to be broken down into individual events and discretely examined.
The 63 kDa lysate band is bovine albumin, which does not bind to heparin and therefore provides a control of immunoprecipitation specificity.
Negative control of reaction specificity was conducted with omission of primary antibodies.
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