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The file " GSE40115- GPL15931_series_matrix.txt" with the normalized log2 ratios (Cy5 sample/controltrof) of probes was used.
The normalized log2 ratios (Cy5 sample/controltrof) of probes mapping to the same gene were averaged to generate independent expression estimates.
The normalized log2 ratios (Cy5sample/Cy3 control) of probes mapping to the same gene (Entrez ID as defined by the manufacturer) were averaged to generate independent expression estimates.
The normalized log2 ratios (Cy5 sample/controltrof) of probes mapping to the same Entrez gene ID were averaged and median centered to obtain the final dataset.
The normalized log 2 ratios (Cy5 sample/controltrof) of probes mapping to the same gene (Entrez ID as defined by the manufacturer) were averaged to generate independent expression estimates.
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The increasing popularity of microarrays in gene expression experiments has allowed for more precise control of probe design and information on full-length sequences enables probes to be optimally designed with higher specificity.
Transcriptomic analysis was performed on a 90K Tomicroarray0 microarray synthesized using the CombiMatrix platform at the Plant Functional Genomics Center of the University of Verona http://ddlab.sci.univr.it/FunctionalGenomics/. CombiMatrix technology combines phosphoramidite chemistry and semiconductors for the digital control of probe synthesis on the chip surface.
A control set of probes, which did not differ between HPV+ and HPV- FFPE samples, also did not correlate with HPV status in the FF set.
Quality control of the probes (i.e. low intensity, saturation, controls, etc).
Quality control of the probes was also done based on their base composition (BC) score.
Quality control of the probes was done based on the detection p-value calculated with Genome Studio.
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