Here, we show that Cre-activated optogene expression in stem-cell-derived neurons allows for robust optical control of neuronal activity for more than three weeks after activation.
Two different observations suggest that both delivery systems might be under the control of neuronal signals.> First, oligodendrocyte cultures without neurons express MBP, PLP, and galactosylceramide (GalC).
To test for optical control of neuronal output in Drosophila, we expressed ChR2 in motor neurons using the D42-GAL4 driver [15].
Remote control of neuronal signaling.
Light-activated ion channels for remote control of neuronal firing.
Rogan, S. C. & Roth, B. L. Remote control of neuronal signaling.
Remote control of neuronal activity in transgenic mice expressing evolved G protein-coupled receptors.
Transcription-dependent and -independent control of neuronal survival by the PI3K-Akt signaling pathway.
Arenkiel, B.R., Klein, M.E., Davison, I.G., Katz, L.C. & Ehlers, M.D. Genetic control of neuronal activity in mice conditionally expressing TRPV1.
Alexander, G. M. et al. Remote control of neuronal activity in transgenic mice expressing evolved G-protein-coupled receptors.
Andrasfalvy, B.K., Zemelman, B.V., Tang, J. & Vaziri, A. Two-photon single-cell optogenetic control of neuronal activity by sculpted light.
