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It has several fundamental uses in metabolomics including experimental design, quality control of experiments, the selection of appropriate statistical methods, and the determination of uncertainty in results.
To gather meaningful expression data, it is crucial to develop standardized approaches for vigilant study design, controlled annotation of resources, careful quality control of experiments, robust statistics, and data registration and storage.
340 Animals (Control of Experiments) Ordinance and Regulations, and all relevant legislation and Codes of Practice in Hong Kong.
Animals (Control of Experiments) Ordinance [Cap 2].
Animals (Control of Experiments) Act (Cap. 71 03).
JP amplified the clones for the microarray and performed many of the hybridizations and the initial quality control of experiments.
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To normalize the amount of cDNA in each sample, the housekeeping gene aldehyde-3-phosphate dehydrogenase (GAPDH) was quantified on the control of experiment with specific primers (forward: 5′-TGTTGCCATCAATGACCCCTT-3′; reverse: 5′-CTCCACGACGTACTCAGCG-3′); the amplicons were 202 bp.
'Control': average of experiments with UTR-SNPs not associated with cancer phenotype (n = 358, 10 independent control trials).
Since the behaviour of the controls of Experiment 1 and of the 'Light Group' of Experiment 2 was determined by last light exposure (phototropism), the question was whether the fan-exposed experimental group could counteract the phototropic effect.
Therefore, separate gene sets were generated of the differentially expressed genes of respectively IM treated animals (n = 3) and OS treated animals (n = 3) versus the controls of experiment 1 as well as the controls of experiment 3.
GSEA analysis showed significant enrichment of this gene set in DHEA IM and PO treated animals when these were compared with the controls of experiment 1 (Table 2).
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