It has several fundamental uses in metabolomics including experimental design, quality control of experiments, the selection of appropriate statistical methods, and the determination of uncertainty in results.
To gather meaningful expression data, it is crucial to develop standardized approaches for vigilant study design, controlled annotation of resources, careful quality control of experiments, robust statistics, and data registration and storage.
In addition, Bourgon et al. demonstrate that non-independent filters for which dependence exists between the filter and test statistic (e.g. making use of condition labels to filter genes with average expression in at least one condition less than a given threshold), can in some cases lead to a loss of control of experiment-wide error rates.
JP amplified the clones for the microarray and performed many of the hybridizations and the initial quality control of experiments.
The animal work was performed according to the Animals (Control of Experiments) Ordinance (Hong Kong) and followed the relevant university guidelines.
According to the Animals (Control of Experiments) Ordinance, Chapter 340 (Department of Health, Hong Kong SAR), there is no requirement for permission to perform experiments involving mussels (invertebrate).
Experimental procedures were carried out by following the protocols approved by Animal Experimentation Ethics Committee of the Chinese University of Hong Kong and Animal (Control of Experiments) Ordinance from Department of Health, Hong Kong SAR; and Institutional Animal Care and Use Committee of the University of Alabama at Birmingham and Johns Hopkins University.
Another gene-targeting system that has provided investigators with extraordinary control of experiments to determine gene function in living organisms is called the Cre/loxP system, which already was alluded to earlier in this article.
Therefore, separate gene sets were generated of the differentially expressed genes of respectively IM treated animals (n = 3) and OS treated animals (n = 3) versus the controls of experiment 1 as well as the controls of experiment 3.
GSEA analysis showed significant enrichment of this gene set in DHEA IM and PO treated animals when these were compared with the controls of experiment 1 (Table 2).
