A control consisting of empty vector was used as a negative control.
Conversely, enforced over-expression of FXYD6 in SMMC7721 cells (Fig. 2E) increased migration and proliferation of these cells, compared with control transfectants of empty vector (Fig. 2F and 2G).
In the experiment shown, the mock-transfected cells were simply treated with the transfection reagent, but similar results were obtained when the negative control consisted of empty plasmids, or plasmids expressing other proteins such as GFP (not shown).
As compared with the control group of empty vector (EV, ROSS level significantly increased 69.2% in U87 cells transfected with G72 plasmids.
On Saturday, an armed group hostile toward the federal government left a protest in Burns and took control of an empty building at the Malheur National Wildlife Refuge.
After attracting funding from a Jersey-based social investor, Steinbeck Studio, which also brought Assemble on board, the community land trust formalised its plans and took control of 10 empty properties from the council last year.
A group of gunmen seized control of an empty federal building in remote Burns, Oregon, on Saturday, announced they planned to occupy the facility for "years" and called for "patriots" to join them -- and bring more guns.
In addition to the control of an empty cage with no recording it was necessary to also use native controls, making it possible to test for the strength of any effect of neophobia or habituation to the parakeet.
Central signals may be as important as peripheral signals in the postprandial control of gastric emptying.
The mannitol challenge (Pharmaxis Ltd ,Frenchs Forest, NSW, Australia) test protocol consisted of inhalations of control (empty capsule) and increasing doses of mannitol to a maximal cumulative dose of 635 mg, per standard guidelines [ 152].
