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After the end of four blocks three control measurements were tested again.
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Correlations between measurements were tested with Pearson correlation test.
All measurements were tested in triplicate.
The measurements were tested for reliability (one-week test-retest).
Kinetic measurements are tested by simulation method.
Control measurements were performed in replicates at the beginning and end of the test.
Control measurements were first recorded.
Scaling parameters and probe quality control measurements are provided in Additional file 8. To test whether SSMIs provide more biologically reproducible results than corresponding intensity scores, we repeated all of our quantitative assessments (see Figures 2 and 3) using SSMIs for the set of optimal probes chosen by RNA-Seq (see Figure 4).
Differences between patients and the population controls were tested by the independent samples t-test and between the measurement points by the paired samples t-test.
Measurement data were tested for normality.
Controls containing the broth were tested concurrently.
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