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Non-music control (labelled 'Control': red line), music audio-playlist groups (irrespective of rhythmic auditory stimulation) (labelled 'Playlist': green line).
Non-music control (labelled 'Control': red line), music audio-playlist without RAS (labelled 'Playlist': green line), music audio-playlist with RAS (labelled 'Playlist + RAS': purple).
The results were analysed by obtaining the log2 ratios of the case (labelled with Cy-5) compared with the control (labelled with Cy-3).
Each real-time PCR reaction was carried out with fluorescent gene-specific primers and HPRT primers as an internal control labelled with FAM Applied Biosystemss).
The non-transformed breast cell line MCF10A engineered to over-express EphB4 under control of the constitutive CMV promoter was used as a positive control (labelled +ve in Figure 3).
Labelling efficiency was determined with a dot blot with control labelled RNA (DIG labelling kit; Roche).
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I think the government is out of control; label me what you want.
Primer probe numbers were catalog number Hs00266705_g1 for human GAPDH Life Technologiess); catalog number 4333760F for 18s eukaryotic endogenous control labeled with FAM probe (Applied Biosystems).
For each drug, four samples were run in parallel: labeled control, unlabeled control, labeled sample, and unlabeled sample.
As a control, labeled rCR2 or K41E CR2 was also added to PBS at 1∶10 v/v.
Ribosomal specific probe pTa71 [45] was used as control, labeled by nick translation using biotin-dUTP or digoxigenin-dUTP.
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