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All samples were normalized to 18S gene expression (18S rRNA Control kit, Eurogentec, Seraing, Belgium).
Subsequently, 15 µg of purified cRNA was fragmented using the GeneChip® Eukaryotic Hybridization Control Kit (Affymetrix).
Control kit DNA and control total spleen cDNA were used as positive controls (far right).
Furthermore, as a positive control, the Duolink control kit contains two primary antibodies targeting different epitopes of chicken TK1 protein.
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Although the inhibitor prevented proliferation of control Kit-dependent F-36P leukemic cells in a dose-dependent manner [20], it did not show any effects on GS cells (Figure 2A and B).
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Labeling efficiency was measured by instant thin layer chromatography (TLC) using the Tec-Control kit (Biodex Medical Systems, Shirley, NY, USA).
Quality control was maintained via the CellSearch® CTC Control Kit used to standardize reagents, instruments, and operator technique.
In this experiment, Poly-A RNA Control Kit and the One-Cycle cDNA Synthesis kit were applied.
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