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The perfect match reaction mixture signal emission intensities were all significantly greater than the combined fluorescence signals emitted by target BG genomic dsDNA and YOYO-1 control intensities and probe and YOYO-1 control intensities (Supplemental Table S1).
For detection of virulence gene orthologs in organisms other than the sources of probe target sequences, a more stringent threshold defined as the 99th percentile of the random control intensities was used.
Background noise of each experiment was evaluated by computing the standard deviation of negative control intensities.
Data were presented with reference to control intensities of β-actin.
Agilent spiked-in control intensities were used to identify the best normalization procedure for each dataset.
Track 7 shows log2 fold changes between the ChIP and control intensities averaged across three replicates.
Similar(42)
However, since there is no shape control, intensity-based techniques may result in leakage of the seeded area or rough edges.
EAAC (200 µg/mL) produced suppression on PI3K and the phosphorylated of Akt level about 85.5 and 68.4% compared to control intensity at 24 h.
The percentage relative fluorescence intensity of in G14-GAL4>VAPwt and G14-GAL4>VAPP58S was calculated by normalizing the control intensity to 100 percent.
We also compared dingo control intensity, scent-marking, howling and abundance with both average annual rainfall and recent rainfall (accumulation of one year prior to the study).
Samples with low bisulphite (BS) conversion efficiency (BS control intensity values <4000) were excluded, as well as other outliers that we detected using boxplots of total intensity I = U+M values and histograms of β-values.
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