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The 18S rRNA gene was used as an internal control gene in RT-PCR.
The 18SrRNA gene (18Sr RNA-F: 5′-CGGCTACCACATCCAAGGAA-3′, 18Sr RNA-R: 5′-GCTGGAATTACCGCGGCT-3′) was used as internal control gene in all qRT-PCR reactions above (Sun et al. 2010).
Based on these, we chose GUS as control gene in this study.
The latter gene was also used as a control gene in our study.
In any case, cyclophilin was used as charge control gene in all samples.
FIGLER 9 (Pal) served as a negative control gene in these experiments, as it was previously shown to be retina-specific [ 16].
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After identifying TFs potentially regulating a maximum number of OC genes, we looked for TFs uniquely regulating estrogen controlled genes in groups 1 and 3.
Our objective was to identify endogenous control genes in circulating neutrophils isolated from cows during the peripartum period.
Thus, β-2 microglobulin and 18S rRNA are suitable internal control genes in quantitative serum-stimulation studies, while β-actin and GAPDH are not.
Three genes CREBBP, EXT1 and EP300 were used as control genes in each assay.
Real-time PCR amplification detected the expression of 80/92 (87%) target genes and all four endogenous control genes in both the CLL and the normal control patient cohorts.
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