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16S rRNA was used as an internal control gene for normalization.
GAPDH was used as housekeeping control gene for UPL based Taqman assay (Roche), as per manufacturer's instructions.
GAPDH was used as an endogenous control gene for normalization of the amount of RNA added to the reactions.
An appropriate control gene for comparing human and chimpanzee expression across multiple tissues was defined by steady expression between and within species as well as across tissues.
The second most down-regulated gene (Gprasp1) was chosen as a control gene for following assays (Mecp2 was not appropriate as it was missing from KO mice) (Table 2).
Human fetal brain (HFB) was used as a positive control for Hh pathway expression, and â-actin was used as a control gene for experiments with cell lines and HFB with and without reverse transcriptase.
The ey gene is a master control gene for eye morphogenesis, because the Ey isoform of the gene has the ability to induce ectopic eye structures in several imaginal discs [42], [43].
These results show for the first time that a splicing factor, namely, B52/SRp55 directs eye size through production of two alternatively spliced isoform of Eyeless a master control gene for morphogenesis.
In this work, we show that B52, an SR protein splicing factor, controls the production of two alternatively spliced isoforms ey and ey (2a) of eyeless, a master control gene for eye morphogenesis.
These data on δ-ENaC clearly show that it can be considered a control gene for our studies, especially since the encoded ion channel is activated by protons (20), and it also proves that the tissue obtained from Patient 8689 contained taste cells.
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Otherwise, the high frequency of virulence implies that there is little obstacle to Z. tritici mutating to virulence on Stb genes which control gene-for-gene interactions.
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