To control for strain variation, these transformations were repeated using MAX Efficiency® Stabl2™ competent cells (Invitrogen).
If not, how do the authors control for strain specific variations?
These results underscore the importance of experimental designs and analytical approaches in expression profiling studies that control for strain effects in animal models and genotypic heterogeneity in patient populations.
Ampicillin (Sigma®) and Amphotericin B (Sigma®) were used as positive control for bacterial strains, and fungal strains respectively while acetone was used as the negative control for both.
The ABR data were analysed for differences between genotypes using a general linear model, controlling for strain background and stimuli.
Of the 15,304 protein coding genes analyzed, 61 showed significant differences between the sexes after controlling for strain differences.
Partial correlations were performed to assess relationships between the stimulatory and rewarding effects of cocaine while controlling for strain effects.
When we controlled for strain lineage and refugee status, katG S315T was inversely associated (OR 0.28, 95% CI 0.09 0.89, p = 0.03) with sputum smear positivity (Table 3).
Females spent significantly more time stationary than males in the open field test (F = 8.413, p = 0.005, Figure 4) after controlling for strain differences.
Consequently, by controlling for strain differences by using littermates from each Snap-25 cross throughout, our study demonstrates that a comparison of the behavioural data from independent mutant lines requires careful consideration of the genetic backgrounds involved (88).
Ampicilinum, ceftazidinum, cefuroximum were used as positive control for bacteria strains.
