Sentence examples for control for purity from inspiring English sources
For every target, amplified PCR products of 10 random positive samples were separated by agarose gel electrophoresis to control for purity and size of the amplicons.
As a control for purity mouse anti-α-tubulin monoclonal antibodies (Sigma Chem Co) and goat anti-laminin B polyclonal antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used.
Nuclear and cytosolic fractions were controlled for purity in Western blots with antibodies directed against the exclusively nuclear or cytosolic proteins Topoisomerase I (nuclear) and Hsp70 (cytosolic) as described previously [22].
We also used MBP as a potential control for the purity of the mitochondrial fractions, but, unfortunately, MBP is drastically reduced in the transgenics, making this protein a less than ideal control.
As a control for RNA purity, mock reverse-transcription mixes (where RT was omitted) were PCR amplified along with samples: no unspecific bands, due to genomic DNA contamination, have ever been observed.
Addition of the test organisms was done in all the wells except for wells of column 11 which contained neat DMSO and broth, this served as control to check for purity.
Since IE2 is not incorporated into viral particles but only expressed in infected cells, this also controls for the purity of our particle preparation.
The total RNA was quantified with a spectrophotometer (NanoDrop, Technologies Inc). and wavelength ratios of A260 nm/280 nm ~ 2 and A260 nm/A230 nm ≥ 2 were controlled for RNA purity.
In the present study, different oat varieties, controlled for their purity and by their distinct protein pattern, were used to examine differences in moAb G12 recognition by ELISA and western blot.
Antibody purification was quality controlled by SDS PAGE for purity and by ELISA.
Microorganisms were cultivated on a mineral medium (MM; Brunner, DSMZ no. 462) and, for purity control, on non-selective Standard I medium (Merck 7881, DSMZ no. 453) or nutrient broth (NB).
