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A common cellular gene (β-actin) was used as a control for integrity of RNA template.
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Total RNA was then quality controlled for integrity on a Bioanalyzer (Agilent Technologies).
RNA concentration was quantified on an ND-2000c UV-Vis spectrophotometer (Nanodrop, Wilmington, DE) and controlled for integrity and purity on a capillary electrophoresis system (Agilent 2100 Bioanalyzer; Agilent Technologies, Santa Clara, CA).
This paper presents a systematic design framework for selecting the sensors in an optimised manner, simultaneously satisfying a set of given complex system control requirements, i.e. optimum and robust performance as well as fault tolerant control for high integrity systems.
Phase contrast images were acquired prior and after each experiment to control for cellular integrity.
To control for the integrity of the DNA, the cellular beta-globin gene was amplified.
As a control for RNA integrity and for loading, GAPDH was expressed equally in all tissues (Figure 1 Panel D).
As a control for DNA integrity, a 124 nucleotide (nt) section of the human beta-globin gene was amplified.
funestus actin gene was amplified as a control for cDNA integrity by using the primers Actin-f and Actin-r (Table S4).
In addition, the concurrent detection of HIV-1 in the seminal plasma samples can serve as a control for the integrity of retroviral nucleic acid.
A p24-specific MAb (183-H12-5C) was used to detect disrupted virions in the pull down assays as a control for virion integrity.
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