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Events for which control for duplicates could not be performed for lack of any record of the patient's complete date of birth (n=91 176, 3.1%) were excluded.
The method used to control for duplicates was validated by comparing the results against data on 30 205 hospitalisations in eight cities for which patient identification codes were available, yielding a sensitivity of 97.88% and specificity of 88.73.
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Identity-by-state values were calculated for each pair of individuals to control for duplicate samples and known relatedness.
It was very good quality, with intention-to-treat analysis of pooled data and controlling for duplicate and language bias.
Quality control included procedures for duplicates and repeating discrepancies.
To control for the latter, duplicate assays were performed in parallel using a different selective media, FOA, where increased silencing could not correlate with decreased colony growth (Fig. 3B, grey bars).
Following recommendations from the authors of the ADTEx manual, we decided to use the ADTEx analysis based on one control sample including duplicate filtering (single control, no duplicates) for further comparison.
The final numbers after exclusions for quality control and duplicates was 6882 in the case group and 6316 in the control group.
During sample analysis, each batch was prepared to include a reagent blank to control for background contamination and duplicate spiked samples (0.25 ng g−1) to confirm OTA recovery of 100% ± 33%.
In every 96-well plate for assay, there is one well for blank control and five wells for duplicate check on five samples for internal quality control.
Further negative controls were duplicates for each drug of drug-only wells without cells in each plate.
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