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In all assays conversion of dexamethasone (Dex) and 11-dehydrodexamethasone (11-DHDex; 40 μM) was used as a positive control for confirmation of 11β-HSD isozyme activity [28].
The assay consists of two internal controls (a conjugate control for confirmation of the colorimetric reaction used to visualise bands and an amplification control to ensure that nucleic acid amplification reaction has occurred) plus a control for each gene locus (gyrA, rrs, embB).
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As controls and for confirmation, nuclear extracts from parallel cultures were analyzed by immunoblotting (Figure 3B).
In brief, 130 individuals of cases and controls were sequenced for confirmation of the rs7044343 and rs10975514.
A total of 84 gene targets (and the 18S rRNA housekeeping control gene) were chosen for confirmation of expression in patient samples, several assessed with redundant probes.
Several positive and negative control loci were used for confirmation of IP efficiency before hybridising to microarrays (Supplementary Figure 1A).
Those who were suspected of having leprosy were referred to a senior leprosy control officer and a doctor for confirmation.
Instrumental techniques such as liquid chromatography (LC) coupled to UV- VIS spectroscopy or mass spectrometry (MS), are widely used in food control for either screening or confirmation of positive findings of less specific test within the following studies: Hermo et al. (2008), Li et al. (2012), Boix et al. (2014), Hou et al. (2014), Martins et al. (2014), and Cepurnieks et al. (2015).
Measurements for standards, controls and serums were repeated for confirmation.
Uncertain cases were referred to the leprosy control officer or DBLM medical officer for confirmation.
Ten per cent of all samples, and all positives, were sent to the University of California Chlamydia Laboratory for confirmation analysis (quality control).
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