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Myostatin induced a ~5-fold increase in luciferase accumulation in control (Empty Vector) cells (Fig. 5a).
(h) Growth curves of Hep3B cells stably expressing FLAG-PKM1 (pWPI-FLAG-PKM1) or control empty vector (pWPI).
Bottom: Enrichment of CBX6 and H3K27me3 at the BST2 promoter in control (empty vector) and CBX6-overexpressing cells.
HCT116 PTPRS KO cells were transfected with a C-terminal FLAG tagged PTPRS or control empty vector.
qRT-PCR analysis of BST2 mRNA levels in control (empty vector) and CBX6-overexpressing cells using GAPDH for normalization.
Mr. Rumsfeld must persuade Pakistan to seal its side of the Line of Control, empty terrorist camps on its territory and close staging areas and infiltration routes.
As a control, empty plasmids were transfected instead of the LPS receptor mix, alongside the NF-κB-luciferase reporter plasmid (Figure S3).
Right panel: Western blot analysis of EZH2 and CBX6 protein levels in control (empty vector) and EZH2-overexpressing cells using GAPDH as a loading control.
Control (empty vector) and CBX6-overexpressing MCF-7 cells were seeded into 6-well plates and synchronized via starvation (without serum) for 24 h.
The volcano plot shows statistical significance (−log10 (P-value) plotted against log2 fold change of genes for CBX6-overexpressing cells against control (empty vector) cells.
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The specific binding profiles were obtained after subtracting the response signal from the control empty channel and from blank-buffer injection.
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