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During the experiments 0.1, 1.0 and 2.0 µM selenocystamine concentrations, cell growth remained similar to the control cultivation that had no selenocystamine addition, without a lag phase at the beginning of the cultivation.
These DCW, lipid and GLA values were respectively 25.7%, 19.6% and 60.9% higher than those obtained in the control cultivation experiment at the standard constant temperature of 28 °C.
Particularly, the fungal morphology could be grouped into either (a) thick mycelial clumps (aggregated or entangled filamentous form of the fungus) as observed in control cultivation; (b) loose or freely suspended mycelia as observed with cultivations in presence of additives (such as glycerol and calcium ions); and (c) pellets as observed at acidic pH cultivation conditions.
The secretome extracted from control cultivation on maltose (referred to herein as PcoMaltose) was also analyzed for comparison.
Interestingly, the energy charge was higher 1 hour after pulsing with inhibitors in the glucose consumption phase than in the control cultivation, which resulted from an increased intracellular concentration of ATP.
Almeida et al. [ 23] supplied 2 gL-1 HMF initially in an anaerobic batch cultivation, which resulted in decreased xylose consumption rate, decreased xylitol yield and increased acetate yield compared to the control cultivation without inhibitors.
Similar(53)
A partial least-squares calibration model, relating mid-infrared spectral features with fructose, ethanol, acetate, gluconacetan, phosphate and ammonium concentrations has been designed to monitor and control cultivations of Gluconacetobacter xylinus and production of gluconacetan, a food grade exopolysaccharide (EPS).
The ATP yield in control cultivations was 11.0 (±0.48) g DW (mmol ATP -1 while it wATP -14 (±0.14) g Dwhileol ATP)-1 when furan aldehydes had been added.
No significant differences were observed in the specific production rates of glycerol, pyruvate and succinate between step-changed and control cultivations.
Transcriptome profiling followed by MIPS functional enrichment analysis of up-regulated genes revealed that the functional group "Cell rescue, defense and virulence" was over-represented when inhibitors were present compared to control cultivations.
The intracellular concentrations of the reduced redox cofactors NADH and NADPH decreased from 0.36 to 0.15 μmol (g DW -1 anDW -1m 0.47 to 0.07 μmol (g DW)-1, respectively, 1 hour ander pulsing ofromhibitors, compared with the control cultivations.
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