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The positive control contained E. coli IF1, IF2 and IF3 but no mtIF2.
A minus reverse transcriptase (-RT) control contained RNA that had not been treated with reverse transcriptase to confirm the absence of DNA in samples.
A negative control contained secondary antibodies only.
An "abiotic" control contained 15 L of filtered (0.45 μm) wetland water.
Tissue culture polystyrene plates (TCPP) used as control contained the same number of cells.
Here, the treated samples had an average of 3.7 × 107 CFU/g, while the control contained 8.75 × 107 CFU/g.
The blank contained calf thymus DNA only and the control contained all components except MT-I or MT-II.
In addition, the negative control contained only medium, while the positive control was medium containing 5% DMSO.
The positive control was used as an antibiotic (Nalidixic acid 10 µg/mL) and negative control contained only inoculated broth.
Negative control contained all components except compounds, whereas positive control galanthamine (10 μg/mL) was used in the assay as standard cholinesterase inhibitor.
The background control wells contained only medium, while the positive control contained only the target cells and medium without the effector cells.
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