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Although apoptosis is the primary form of programmed cell death used to control cell viability, cell death can also be associated with the presence of autophagic vacuoles, indicative of autophagic cell death.
Taken together, these results reinforce the specificity of action of the different MT isoforms and suggest that MT2A isoform rather control cell viability than invasiveness or motility, at least in our osteosarcoma models.
After the treatment of samples and positive control, cell viability was detected and a cell growth curve was graphed.
We set about to find the optimal [CdCl2] that would elicit the RNAi response without affecting the control cell viability or causing the cells to rupture.
The values are expressed as percentage of control cell viability.
As a control, cell viability was analysed by trypan blue exclusion assay according to the manufacturer's instructions (Sigma-Aldrich Chemie GmbH, Munich, Germany).
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We therefore hypothesize that FAK expression might play a key role in controlling cell viability for cells cultured on CNT scaffolds.
In vitro, we further confirmed the association between SNAT1 expression and Akt activation, which controlled cell viability and colony formation.
In control cells, viability was increased 2.8 fold in medium containing 0.5% FCS and HRG compared with medium supplemented with 0.5% FCS only.
The GenMAPP analysis by pathways revealed that felodpine can modulate cell cycle, DNA replication and cell adhesion (Figure 7), thereby supporting mechanisms by which felodipine could control cell proliferation and viability.
As compared to control cells, the viability of COX-deficient antimycin-treated cells was reduced to 70 80% for SURF1, COX10 or COX15 mutated fibroblasts and 85% for COX-deficient HEK cells, while antimycin did not affect the viability of control cells (Table I).
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