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The number of migrated MDA-MB-453 cells was reduced to 53.2% after transfection with BCL6 siRNA (P < 0.01; Figure 3c), and was 66.9% lower than control cell for cell invasion (P < 0.01; Figure 3c).
Representative images of drug-exposed cells exhibiting spectra from each of the three groups are also shown in Fig. 5. Also shown is an image of an untreated control cell for comparison.
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HSP90 or control vectors were transfected into PI4KIIα siRNA-treated or control cells for 36 h, and indicated proteins detected by Western blot, with β-actin serving as control.
IC50 values (concentration required to reduce cells viability by 50% as compared to the control cells) for each sample was calculated by curve fitting of the cell viability data.
However, at 12 h, values were significantly higher than unexposed control cells for all the CYPs except for CYP2B1.
The relative quantitative method (DDCT) was used to evaluate gene expression in experimental and control cells for each gene examined[69].
This metalloprotease inhibitor was added to the conditioning medium of both BACE1 and control cells for two reasons.
CHO-K1 and HeLa cells expressing recombinant proteins were incubated with 50 µM 2-BP or vehicle (DMSO) for control cells for the indicated times.
We included unstimulated control cells for each of the time points to prevent bias with respect to loading and quenching of the dyes.
In contrast, DRB-treated populations steadily increased alongside control cells for only the first 3 4 hours, after which time nuclear labeling plateaued and diverged from that of control cells (Figure 1C, squares).
A significant difference was noted when the densitometry data were compared with control cells for 7.13, B128, J245, J243, J198, J178, J166, and J54 strains in AGS and MKN45 cells (*P<0.01); the exception was strain J117 in both cell types.
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