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I am currently studying the molecular interactions which control cell death.
We reversely transfected LNCaP or 22RV1 cells (2.5 × 103 per well) with control, cell death, RGS17 siRNAs using HiPerFect transfection reagent (Qiagen).
These results indicate that the interactions of the Bcl-2 network can generate a good switch to control cell death in a certain window of parameter variations.
However, numerous studies have shown that E2F activity could also promote the expression of genes that control cell death, differentiation and development programs [1].
Specific cell death was calculated as (% of drug-induced cell death − % control cell death)/(1 − % control cell death) as described (Lajmanovich et al, 2009).
Control cell death was determined in un-transfected HL-1 cells incubated in oxygenated normoxic buffer for 13 h.
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Functional delivery efficacy was detected using a positive control cell death-inducing siRNA, with which successfully delivered siRNA would result in cell death.
And Robert Horvitz, of the Massachusetts Institute of Technology, was honored for identifying various genes controlling cell death.
Ceramides are also powerful intracellular signalling molecules controlling cell death, growth and differentiation.
In a series of experiments beginning in the 1970's, Dr. Horvitz used C. elegans to determine the existence of a genetic program controlling cell death.
Horvitz's research focused on determining if a specific genetic program controls cell death.
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