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A CHO cell line stably expressing the reporter gene secreted placental alkaline phosphatase (SPAP), under the transcriptional control of a six-cAMP response element (CRE) promoter (CHO-CS cells) was used as a control, as appropriate.
CHO-K1 cells stably expressing the secreted placental alkaline phosphatase reporter gene under the transcriptional control of a 6-cAMP response element promoter (CHO-CS cells) were used as a control, as appropriate.
Vehicle control (as appropriate).
The densitometric signal was quantified using ImagePro Plus software version 4.0 (Media Cybernetics, Silver Spring, MD, USA) and normalised to that of loading control as appropriate.
MDA-MB-231, MDA-MB-453, and HeLa cells were co-transfected with pMirTarget-MST4, miR-4728-3p mimic, mimic negative control, and/or pMirTarget-Vector control as appropriate using Lipofectamine 2000.
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Either uninfected mosquitoes or HepG2 cells were used as negative controls, as appropriate.
Independent samples t-tests or Mann-Whitney tests were used to compare means or medians between cases and controls as appropriate.
If more than one infected wound from a patient met the criteria for inclusion in the study population (i.e., SSTI diagnosis and isolation of specific bacterial organisms), the infected wounds were independently treated as cases and controls as appropriate.
These indices were expressed as the quotient of total individual scores for respective countries and the total number of melanoma probands or control subjects, as appropriate.
Prior to reverse transcription, any residual DNA was removed using DNAseI (Epicentre, Madison, USA) in accordance with the manufacturer's instructions, with PCR amplification controls performed as appropriate.
In determining the effect of UIA treatment on cognition, however, a comparison between patients and controls is not as appropriate as one between patients' performances before and after treatment.
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