Fixed cells were then permeabilized, stained using Nanog Alexa-Flour or Isotype control and visualized by FACS on FACSCalibur using the CellQuest software (both from BD Biosciences, San Jose, CA, USA).
Nested PCR-EIA negative samples were re-tested after adding 10 ng internal inhibition control and visualized by gel electrophoresis to assess possible inhibition.
Our algorithm updates the dynamic ink volume using a hybrid grid-particle method: the fluid velocity field is calculated with a low-resolution grid structure, whereas the highly detailed ink effects are controlled and visualized with the particles.
Blots were then stripped (with 2%SDS, 12.5% Tris pH 6.8, 0.8% β-mercaptoethanol) for 45 minutes at 50°C, re-probed with antibodies used for loading controls and visualized by chemiluminescence.
To examine this possibility, HeLa cells were co-transfected with MT-RFP and either MULAN or the vector control, and microtubules were visualized by staining with α-tubulin antibody.
Being my second time through the series of call rooms I was able to control my thinking and visualized the start of the race.
Figure 1B shows affinity purified plastid proteins after incubation with rbcL or control RNAs, separated and visualized by SDS-PAGE.
To further examine the effect of PKD on autophagy, 293T cells ectopically expressing PKD-GFP, or GFP as control, were fixed and visualized by electron microscopy (EM).
Genes that differed significantly between treatment and control were analyzed and visualized to see the results of GO term enrichment using the classic algorithm and Fisher's exact test in the Bioconductor topGO package (http://www.bioconductor.org/packages/2.12/bioc/html/topGO.html).
That is, with a 4/1000 chance of coincidence, the population they tested showed a small ability to control computers with their minds, and visualize things in a different location.
