Sentence examples for control and tester from inspiring English sources

The PCR not only exponentially amplifies the control and tester DNA but also separates them from each other.

The adapter-linked control and tester DNA are reassociated through mixing at a ratio of 1 1, denaturing and annealing.

The DNA reassociation results in the quadratic magnification of expression ratios for the up- and down-regulated genes in control and tester samples.

With indirect labeling, aminoallyl-dUTP is incorporated into control and tester DNA with PCR of 3 cycles after the reassociated DNA templates are amplified.

Briefly, the control and tester cDNA's are digested with Taq I restriction enzyme, then ligated with the CT and TT adapters, respectively.

For example, the 3DNA fluorescent dendrimer probes (Genisphere, Montvale, NJ) can be attached on the CT and TT primers which then are used to amplify the control and tester DNA's.

With either approach, control- and tester-derived targets are co-hybridized to the microarray and control- and tester-derived signals are compared, often by computing the Log Ratio (LR) = log2 tester signal/control signal).

With direct labeling, Cy3-dCTP is incorporated into control and Cy5-dCTP into tester DNA during the PCR amplification of the hybridized DNA templates.

In brief, mRNAs from the last step for both control and treated samples were designated as driver and tester, respectively.

Poly(A+) mRNA from aMPV-infected and control cells were also used conversely as driver and tester samples, respectively (Reverse SSH library).

Subtractive hybridization was performed using five-times more driver (control) than tester (infested) cDNA.