Sentence examples for contribution of polymerase from inspiring English sources

To establish the causes of uveitis in immunocompromised patients and to determine the contribution of polymerase chain reaction (PCR) and Goldmann-Witmer coefficient (GWC) analysis of aqueous humor in patients with an infectious etiology.

Plasmid prepared from two to four colonies from each PCR product was sequenced separately to minimize the contribution of polymerase errors to sequence variation.

To determine the contribution of polymerase strand slippage errors to the population polymorphism incidence values, we measured the production of unit-based indel errors within mono- and dinucleotide TRs.

We experimentally examined the contribution of polymerase slippage errors to dinucleotide TR mutability for (GC) n, (AT) n, (TC) n, and (AG) n motifs ranging from 2 to 14 repeats, inserted within the same sequence context of the HSV-tk gene.

We used our established in vitro HSV-tk mutagenesis assay (Messier et al. 1996; Eckert et al. 2002; Eckert and Hile 2009; Kelkar et al. 2010) to evaluate experimentally the specific contribution of polymerase strand slippage errors during DNA synthesis to the observed mutational behavior of TRs in the human genome.

To assess the direct contribution of polymerases from virus types 1 and 2 to mutation frequency, recombinant HSV expressing a type 2 Pol within a type 1 viral genome were generated by marker transfer and examined along with a panel of other HSV isolates in two mutagenesis assays.

Moreover, we used an in vitro assay to assess the contribution of DNA polymerase strand slippage errors to polymorphism incidence for mono- and dinucleotide TRs, and thus shed light on mechanisms underlying the mutational behavior of TRs.

In these organisms, among the most evolutionarily divergent eukaryotes for which there is significant molecular information [ 13], the contribution of RNA polymerase II promoter activity to regulated gene expression is unimportant [ 14].

To assess directly the contribution of viral polymerase activity (rather than basic processes such as RNP formation) on SUMO redistribution, we also tested inactive RNPs in this assay, either by omitting an essential polymerase component or by substituting in a mutant polymerase.

Specifically, this study evaluated the contribution of type 2 polymerase to mutation frequency when measured by the tk mutagenesis and non-HSV DNA mutagenesis assays.

This set of experiments also tested potential contributions of small RNA polymerase-associated hydrophobic molecules (such as lipids) to RNA polymerase oligomerization.