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To understand how the N-Linker contributes to assembly of tetrameric holoenzymes, we engineered a monomeric RIα that contains most of the N-Linker, RIα 73-244), and crystallized a holoenzyme complex.
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Contributed to assembly, annotation, and summary of sequence quality.
At hair follicles, these filaments may contribute to assembly and maintenance of lanceolate ending-TSC-epithelial cell complexes.
Sequencing errors, sequence variants and repeats cause branches in the de Bruijn graph contributing to assembly difficulty (Fig. 1).
First, repetitive DNA is a known difficulty for assembly of short sequences into contigs, and the prevalence of retrotransposon sequences could contribute to assembly difficulties.
The Silene genome is known to include a large number of repeated elements [ 32- 34] and we had to filter out such elements because they contribute to assembly problems.
A 2′-O-methyl oligonucleotide complementary to the 5′ end of U1 snRNA reduced ED-A formation, showing that U1 snRNP also contributed to assembly (data not shown).
Besides, the homologs also contained N-terminal PapD-like domains, which can contribute to assembly of surface organelles involved in cellular attachment [ 36].
This high repeat content also may have contributed to assembly problems in some clones, as almost all contained missing sequence and multiple scaffolds.
Spring-like mechanisms contributing to assembly of active recombination complexes also have been proposed for the Tn10 transpososome (Chalmers et al., 1998).
Our genetic analyses suggest that all MEGs contribute to assembly and disassembly but to different extents, with MEG-3 and MEG-4 together making the most important contribution to assembly.
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