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While HV− cells in 2i displayed high-level epiblast contribution, HV+ cells from 2i contributed efficiently to the epiblast and to all extraembryonic lineages (i.e., exhibited totipotent properties).
Remarkably, ES cells carrying two copies of the mutant Sp1 allele expressing a truncated protein lacking the entire DNA-binding domain (Sp1−/−; Fig. 1A, supplementary material Fig. S1A) were indistinguishable from wild-type cells in culture, and contributed efficiently to chimeras until E9.5, after which contribution of the mutant cells rapidly declined to undetectable levels (Marin et al., 1997).
When naïve-like ESCs were injected into preimplantation rabbit embryos, although they contributed efficiently to forming the inner cell mass of blastocysts, no chimeric pups were obtained.
The mES cells that stably expressed EGFP following M9-assisted lipofection yielded > 66% chimeric mice (8 of 12) and contributed efficiently to the germline.
The tests showed that MAC-MFC generated stable and higher power outputs compared with SC-MFC and each anode chamber contributed efficiently.
The results showed that the adopted in-plant modifications such as installation of water flow meters, spring valves on water hoses, and design of a steam condensate recovery system contributed efficiently in saving water and energy consumption throughout the pasta plants.
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In summary, the results demonstrated that residue 279 of VP2 did not contribute efficiently to cell tropism, replication efficiency, and virulence of IBDV at least in some strains.
We believe that ESCs carrying chromosomal translocations should contribute efficiently to chimera formation if the disrupted genes are not important for embryonic development.
This development will contribute efficiently towards a new robust rational model of general applicability for simplified seismic analysis of tall guyed telecommunication masts.
Highly skilful and competent human capitals are critically needed to accelerate and contribute efficiently towards the development of technology in spearheading Malaysia's transformation agendas.
With physical and biological changes occurring at unexpected pace, marine phycologists should now integrate and join their research efforts to be able to contribute efficiently for the conservation and management of coastal systems.
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