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We propose that interaction of START domains with accessory proteins, via tissue-specific expression and proper subcellular compartmentalization, are likely to contribute to specificity in binding of selected metabolite ligands during the lifespan of the protein within the cells of the host organism.
The other variable positions in the HTH are at positions 5, 7, and 8, and while these may contribute to specificity, there are no clear correlations with site differences.
The observation that miRNAs cause decreases in the abundance of at least some mRNA targets provides a powerful strategy for determining what features in mRNA and miRNA sequences contribute to specificity [11], [33], [34], [36], [37], [39], [40], [42], [43], [45].
This may contribute to specificity of [Mg2+]i-dependent effects in different preparations.
The latter does not contribute to specificity because loss of basal activity indiscriminately slows cleavage of all substrates.
The diversity of core promoters are thought to contribute to specificity of gene regulation in a combinatorial fashion [ 55].
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Identification of several factors contributing to specificity of 3'-UTR target sites has helped improve target prediction methods [ 6].
Detailed comparative structural analysis by crystallographic or NMR-based methods would be required to identify the subtle structural differences contributing to specificity.
Highly conserved residues, however, are not selected by our method since our measure is designed to emphasize on sites contributing to specificity.
In addition, the cell-type specific expression of Importin-α isoforms may contribute to the specificity of the phenotype [53], [54].
In addition to the specificity of the antibody, the short reaction time between antigen and capture antibody might also contribute to the specificity of the assay.
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