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Aortic rings were pre-contracted with 10 μM PE and once a stable contraction was achieved, cumulative concentration response curves were obtained to the relaxatory agonist carbachol (CCh: 10 nM to 30 μM).
Cell cell interactions also influenced the aligned architecture, as extensive astrocyte-collagen contraction was achieved at high (9 12 × 105 cells/mL) but not lower (2 6 × 105 cells/mL) seeding densities.
Because maximum collagen contraction was achieved in response to 1 ng/ml of TGF-β2, all subsequent experiments used this dose.
At this morpholino dose, knockdown efficacy was estimated at close to 80% and nearly complete cessation of cardiac contraction was achieved in >90% of wild-type embryos (data not shown).
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A simple finite element model confirms that faster contraction is achieved with increased stiffness.
Notably, in our model, co-contraction is achieved without changing the standard energy based cost function since the uncertainty information is contained in the learned internal dynamics function as a stochastic term.
Active motor threshold was identified in the first dorsal interosseous muscle while subjects provided a gentle background contraction; this was achieved by opposing index finger and thumb.
Maximum isometric contraction torque was achieved by three maximal isometric attempts at 50° for the elbow flexors and 60° for the knee extensors, 17 with 60 seconds of rest between attempts.
In the case of altering stimulus frequency from 0.1 to 5.0 Hz, the steady state contraction of myocyte was achieved (usually after the first 5 6 beats) before PS was recorded.
In the case of altering stimulus frequency (from 0.1 Hz to 5.0 Hz), the steady-state contraction of myocyte was achieved (usually after the first five to six beats) before PS amplitude was recorded.
Once a stable contraction >5 mN was achieved, cumulative concentration response curves were constructed to CBD.
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CEO of Professional Science Editing for Scientists @ prosciediting.com