Exact(31)
In Fraction-I some non-contracting cells were always noticed between the (in time in unison) contracting cells.
With the 2-D cultures contracting cells were present after 1 day, and reached confluency from day 5 on and this was maintained up to 135 days.
These spontaneously contracting cells are autonomously powered with glucose as an energy source without any external power supply or stimulus, unlike conventional micro-actuators/micro-pumps.
Although we detected Ca(2+) transient oscillations in the reprogrammed cells, we did not detect significant changes in resting membrane potential or spontaneously contracting cells.
All rhythmically contracting cells expressed the MHCα::GFP reporter (Movie S1).
Spontaneously contracting cells appeared as clusters in outgrowths from the EBs at day 10 after differentiation.
Similar(29)
At day 8, contracting cell colonies in fields of 3 independent samples were counted from the video record with Image J software (National Institutes of Health, Bethesda, MD, www.nih.gov).
By day 7, the CPCs underwent differentiation-commitment into cardiac cell lineages including cardiomyocytes as indicated by the initiation of Mhc6 expression along with the detection of spontaneously contracting cell clusters.
Nested collagen matrices were prepared by embedding contracted cell-containing matrices (also called dermal equivalents) inside cell-free matrices; migration occurred from the former to the latter.
Thus, the mechanisms that operate to contract cell boundaries and to expand new ones respond differently to epithelial stresses.
Overall, H2O2-treated neurons showed a significant decrease in MAP-2-positive cells, with contracted cell bodies and neuronal processes and predominant nuclear staining (blue, 4′, 6-diamidino-2-phenylindole (DAPI), arrows, Figure 5d).
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