However, these differences occurred at NaCl concentrations that were roughly 10-fold higher than at pH 8. Difference maps at 20 or 100 mM NaCl at pH 4 were largely featureless, except for one comparatively weak pair (6 or 7 contour levels peak-to-trough) at the position of the strongest peaks at higher NaCl concentration.
The number of contour lines (peak-to-trough) was counted for each set of positive/negative difference peaks in the difference maps shown in Figure 5 including maps generated for 50 mM NaCl at pH 8 and 150 mM NaCl at pH4 (not shown, see Supplementary file 1 ) and plotted against the Na+ concentration at pH 8 (A ) and pH 4 (B ).
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To create the pitch contours, initial peak candidates are filtered using a salience threshold and a deviation threshold.
Differences were much weaker (maximally 7 8 contour levels peak-to-trough) and more diffuse than at pH 8 or at pH 4, even up to 1 M NaCl (not shown).
Whilst all three groups displayed a final rise-fall contour, the F0 peak was found to occur much later for the Malay speakers.
Numbers right-adjacent to the contour lines indicate peak release rate and half-duration of the TCR, respectively.
The most dramatic differences from year to year were in spreading pattern, as shown in the contour map of peak ILI activity by week.
Bottom, contour plots of peak release rate (left) and half-duration of the TCR (right), plotted vs coupling distance (horizontal axis) and Ca2+ inflow (vertical axis, normalized to that of single Ca2+ channel).
This benefit is made apparent when a tip creates an intricate contour having multiple peaks and valleys within a single scratch.
