Sentence examples for continuously subsequently from inspiring English sources

To study further the impact of paclitaxel chemotherapy on the expression of miR-375 in cervical cancer cells, SiHa and Caski cells were cultured with 10 nℳ paclitaxel, after 72 h, the paclitaxel was removed and the cells were cultured with complete medium continuously, subsequently the expression of miR-375 was measured by using RT PCR at day 7, day 14, and day 21, respectively.

At least 200 tumour measurements in each of 17 patients with accessible tumours were taken before, and subsequently continuously after the commencement of carbogen breathing for periods of 10 to 30 min. In 12 out of 17 patients studied there was a significant increase in median tumour p02 during the first 10 min of carbogen breathing (range 9 to 1800%).

The waiting time of the packets in the queue continuously increases, subsequently increasing the delay.

The observer design is first carried out in the case where the output measurements are continuously available and subsequently extended to the case where the outputs are available only at (non equally spaced) sampling instants.

Cells were subsequently continuously selected in G418-containing media to enrich for successfully transduced cells.

HIF-1 β is a constitutively expressed subunit, whereas HIF-1 α is translated continuously and degraded subsequently through ubiquitination under normoxic conditions [ 36, 37].

Itch intensity was assessed continuously for 5.5 minutes subsequently.

Such cells were inoculated into NOG-SCID hosts and a continuously growing tumor was subsequently harvested to re-establish in vitro E006AA cultures.

Aphis gossypii were provided by Syngenta (Stein, Switzerland) and were subsequently reared continuously on cotton (Gossypium hirsutum) in a climate chamber at 25±1°C, 70±10% r.h., and a 16-h photoperiod.

The specific enzyme activity increased to a maximum of 1500 IU gDCW-1 after 12 h of induction, and subsequently decreased continuously.

Subsequently and continuously, hCMEC/D3 were perfused with freshly isolated PMN (1 × 10 PMN/mL; suspended in serum free-VascuLife EnGS-Mv cell culture medium) that have been stimulated either with CCM or SSCM for 10 minutes.