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The chimera mice were continuously bred in B6 background and the offspring shows typical Mendalian distribution of +/+, +/− and −/− genotypes (data not shown).
Six control female FVB mice were continuously bred separately.
Worms can be continuously bred in the lab throughout the year using a well-developed culture protocol (Hauenschild and Fischer 1969; a detailed description in English is available at http://www.uni-giessen.de/~gf1307/breeding.htm).htm
The Kyoto-Runx3 KO we sent to Israel in 2010 have been continuously bred for about 10 years, which may have resulted in exon 4 skipping and explain why Levanon et al could not observe LacZ staining in GIT epithelium of Kyoto-Runx3 KO (Levanon et al, 2011).
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Several age-synchronized cohorts (hatched from egg masses laid within 24h), ranging from eggs to 4 months old, are bred continuously.
To maintain maximal ERBB4 expression, transgenic mice were bred continuously for a year after which mammary glands were isolated and analyzed.
The continuous occurrence of immature specimens and pregnant females during the whole study period indicates that M. lusitanicus can breed continuously throughout the year in apple orchards in Asturias.
At lower latitudes many, but not all, species breed continuously.
In the tropics, many amphibians breed continuously or at any time of year.
The values of apparent digestibility performed on sequentially fed hens were similar to values usually obtained on continuously fed hens, as observed in previous studies with similar bred hens.
The fish were bred for at least 6 months, and females and males were continuously maintained in a ratio of 1 2.
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