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By investigating known protein complexes combined with gene expression data, we find that most protein complexes can be formed in continuous time points, and the average overlapping rate of the known complexes during the formation is large.
The correlation between routine coagulation and ROTEM® variables with the severity of organ dysfunction defined by the SOFA score was studied using the nonparametric Spearman correlation (r) with pooled data from the continuous time points.
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At continuous time point, cells were collected and analyzed by immunoblotting and flow cytometry analysis.
According to the just-in-time mechanism, a complex C can be formed in a continuous time point set.
About 63.4% known complexes can be formed in a continuous time point set, while only a small portion of predicted complexes can be formed in a continuous time point set.
C can be formed in a continuous time point set [ S, S+ K], if the boolean function F C C, S, K) is true.
The percentages of complexes which are formed in a continuous time point set in the known complexes and predicted complexes are list in Table 1.
For the complexes which are predicted by clustering methods, only a small portion of them can be formed in a continuous time point set, and the average overlapping rates during the formation are significant lower than that of the known complexes.
Furthermore, we analyze the known protein complexes based on the complex formation model and combined with gene expression data, and find out that most complexes can be formed in a continuous time point set and the average overlapping rate of the known complexes during the formation is larger than 0.5.
Expression levels of those genes were recorded at 72 continuous time-points yielding a [798 genes×72 time-points] matrix (Table S3, supplementary material).
Each time series consisted of continuous time-point measurements acquired for 5 minutes.
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