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Flavanones were extracted from 100 mg powder needle sample in 1.5 mL ethanol 95%% over night at 4 °C under continuous shake (200 rpm).
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Incubation at 56°C with continuous shaking was continued for an hour.
Then, cells were washed and incubation was continued for 15 min at 37°C with continuous shaking.
Fungal cells were cultivated at room temperature (RT) with continuous shaking (150 rpm).
A continuous shaking of the air and earth followed, then darkness, fog, and, later, out of the mushroom cloud, "a shower of most peculiar rain.
Cells were incubated with virus at room temperature with continuous shaking on shaker for 1 h and then incubated at 37 °C for one additional hour.
Remaining supernatants were incubated with continuous shaking in the presence of monomeric avidin beads in Triton X-100 buffer (100 μl/tube) for 1 h at room temperature.
Various concentrations of the cells were seeded onto coated and uncoated polymer matrices in tissue culture dishes without shaking ("static seeding") or continuous shaking ("agitated seeding").
The mixture was incubated at 30 °C under continuous shaking.
Each sample was heated up to 100°C for 2 h with continuous shaking.
The adsorption process was carried out under the conditions of continuous shaking through 24 hours.
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