Sentence examples for continuous pcr from inspiring English sources

Continuous PCR amplification of KIF7 exons from whole blood mRNA was not successful.

After sequencing and assembling the obtained cDNA fragments, one complete open reading frame (ORF) was obtained, and then confirmed by continuous PCR amplification.

In the chip operation, after the continuous flow PCR was achieved in the first microfluidic chip, the product was directly introduced into a hybridization chip integrated with microarray containing the immobilized DNA probes.

Reverse transcription and amplification were performed with a continuous RT-PCR method with the Qiagen OneStep RT-PCR Kit (Qiagen) following the manufacturer's instructions.

ABV RNAs were reverse-transcribed and amplified with a continuous RT-PCR method by using a One Step RT-PCR kit (QIAGEN) according to the manufacturer's instructions.

The direct combination with a continuous-flow PCR system realises an integrated diagnostic device for pathogen detection.

Herein, we successfully developed a microfluidic device which combines high-throughput continuous-flow PCR and DNA hybridization for the detection of various bacterial pathogens.

To our knowledge, our group is the first group to introduce an IR lamp, which costs less than USD $20, into the heating module that has been applied to the design of a continuous-flow PCR device.

Reverse transcription and amplifications were performed in a continuous RT-PCR method by using the QIAGEN OneStep RT-PCR Kit (Qiagen GmbH).

A continuous-flow PCR microfluidic chip was recently developed by employing a water cooling thermocycling within the chip center whereby the sequence of denaturation, annealing, and extension was created due to the forced convection effect and DNA fragments with different lengths (372 bp and 478 bp) were successfully amplified [ 19].

First, a universal JEV-group specific oligonucleotide primer pair designed on the nonstructural protein 5 (NS5) and 3´-untranslated regions (UTR) of WNV (forward primer: 5´-GARTGGATGACVACRGAAGACATGCT-3´ and reverse primer: 5´-GGGGTCTCCTCTAACCTCTAGTCCTT-3´ [ 21]; ) was applied on the RNA extracts in a continuous RT-PCR system employing the QIAGEN OneStep RT-PCR Kit (Qiagen).