Sentence examples similar to continuous passage from from inspiring English sources

Live attenuated variants of Y. pestis were obtained from wild-type isolates by continuous passage on routine bacteriological media at an ambient temperature and consequently used as the LWC plague vaccines.

Tachyzoites of the T. gondii Me49 strain were obtained from the ATCC and subsequently maintained by continuous passage in Vero cells (using RPMI [Gibco] and 2% heat inactivated newborn calf serum [NBS]) at 37°C in a 5% CO2 incubator.

The parasites were maintained by continuous passage in human foreskin fibroblasts (HFFs) as previously described [51].

Toxoplasma gondii parasites were maintained in continuous passage in Hs27 human foreskin fibroblast (HFF) cell monolayers (ECACC number 94041901).

L. major (MHOM/SU/73/5ASKH) was kept in a virulent state by continuous passage in BALB/c mice.

Continuous passage of Mdr2-restored MEFs readily gave rise to established 3T3 cell lines.

Cells were maintained in continuous passage by trypsinization of subconfluent cultures.

A few years earlier, the governing Liberal Party introduced the Continuous Passage Regulations, mandating would-be migrants to the Canadian dominion must arrive there by direct route from their birth or resident country.

After reaching confluence, cells were detached by 0.25% trypsin for 1 2 min at 37°C and replated for continuous passage.

It is reassuring to note fewer variations between the two subcultures from the same laboratory (c and d) which were examined over a period of 5 months in continuous passage.

When confluent, RT4 cells were passaged with 0.05% Trypsin-EDTA for 5 minutes and then plated onto T75 flasks for continuous passage or onto 24 well plates for use in experiments when confluent (approximately 3 to 5 days after passage).