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The houses of TLC trial children were cleaned up by vacuuming, mopping, minor repairing, and paint stabilization to minimize continuous lead exposure (Rogan et al. 2001).
All analyses were conducted using SAS, version 9.3 (SAS Institute Inc ., except for analyses incorporating penalized splines for the continuous lead term, which were completed using R, version 3.0.1 (R Core Team 2013).
In the SRD and MRD parts of the study, continuous lead II ECG monitoring was performed for a minimum of 4 h after dosing and QTc interval was determined from read out of the ECG machine.
For 24-month metal analyses, results for MDI among the subset of 267 children with both 12- and 24-month blood samples were generally consistent with results based on all 24-month samples [ n = 437; mixed-effects model adjusted β, manganese quintile 5 × continuous lead: subset of 267 children, –0.53 (95% CI: –1.41, 0.35); total sample, –0.09 (95% CI: –0.63, 0.45)].
In the subset of children who provided blood samples at both 12 and 24 months (n = 267 vs. total n = 275 for 12-month samples), results from 12-month metal analyses were similar [e.g., mixed-effects model adjusted β, manganese quintile 5 × continuous lead predicting MDI: subset of 267 children, –1.26 (95% CI: –2.17, –0.37); total sample, –1.27 (95% CI: –2.18, –0.37)].
For PDI, however, we observed a negative interaction between 24-month lead and manganese only among the subset of children with both 12- and 24-month blood samples [mixed-effects model adjusted β, manganese quintile 5 × continuous lead: subset, –0.86 (95% CI: –1.66, –0.05); total sample, 0.18 (95% CI: –0.32, 0.68)].
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