The other three sessions employed continuous imaging (8 functional images per block, TR 2.9 s, 23.2 s/block, interleaved slices) to permit the analysis of the time course of activations.
Amplitude changes in SDAs and ARMs were examined over images 2 8 in continuous imaging blocks (Figure 13).
The probe molecules in these experiments tend to degrade or "photobleach" rapidly, limiting researchers to just a few seconds of continuous imaging or a series of images taken seconds apart.
Changes in activation magnitudes within blocks were examined by analyzing images 2 8 obtained during continuous imaging conditions.
Once the image plane was determined, continuous imaging (1 Hz) commenced.
In the current study each subject participated in six functional brain imaging sessions of an intermodal selective attention task that independently manipulated attention as well as tone frequency, intensity, ear of delivery, and image acquisition parameters (sparse vs. continuous imaging).
Two images were acquired per block during sparse imaging sequence and eight images were acquired per block during continuous imaging.
With the advanced methods of digital image processing and optical sensing, it is possible to have continuous imaging carried out on-line in combustion processes.
For most analyses, mean BOLD responses associated with each block were calculated by averaging across both functional images in the sparse sampled blocks and across images 2 8 (i.e., beginning 5.8 s after block initiation) in continuous imaging sessions.
Furthermore extended time-lapse imaging of fluorescently labelled cells can cause significant phototoxicity2, which limits the duration of continuous imaging.
The effects of CsA on microglial speed, Euclidean distance and meandering index was calculated for each 24-hour time-window over the 72 hours of continuous imaging.
