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Males that continued to sire offspring in month 2 were treated in month 3 of breeding with 50 mg/kg BID ("high-dose" regimen), and JQ1 treatment was stopped for these males and controls while continuing to breed to 2 females per cage through 8 months of continuous breeding.
For mating studies, males were pretreated for 6 weeks with vehicle or JQ1 and caged continuously with two females each while continuing 50 mpk QD for month 1 and escalating to 75 mg QD for month 2. The JQ1-treated males that were responsive to this "low-dose" treatment regimen were allowed to recover untreated while continuing to breed to 2 females per cage through 7 months of continuous breeding.
Females were slightly more prevalent than males and an analysis of the sizes of specimens suggested the presence of multiple cohorts and continuous breeding.
Continuous breeding studies and 90-day studies with additional measurements of sperm parameters were similarly effective in detecting compounds which affect male fertility.
Evidence of continuous breeding activity was determined for a high number of species at the three habitats (> 70% lactating and 65% with embryos).
The relatively mild weather conditions in winter and the presence of a permanent grass cover throughout the year seem to be favourable conditions for continuous breeding of the vole.
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In Dielmo malaria transmission is perennial because a nearby river maintains continuous mosquito breeding sites.
Endemic areas are limited to the tropics, where continuous mosquito breeding is possible.
Continuous dahlia breeding worldwide has led to the availability of a countless number of cultivars, many of them showing red hues.
GSK3 knockin mice and matched wild-type mice of mixed C57Bl6, Balb/c, and Ba11 background [27] were generated by continuous inter-breeding of homozygous knockin mice and wild-type mice of the same background.
Many mutant rice varieties with various phenotypes were generated after continuous selection and breeding.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com