Your English writing platform
Discover LudwigExact(39)
A novel approach for the determination of a kinetic model of enzyme deactivation is presented incorporating time and temperature dependence into a single, continuous assay.
Still they are supposed to be simple method (Stuckey et al. 1980), inexpensive, reproducible, and useful in the development of continuous assay programme.
A field portable sample preparation, enzyme extraction protocol and continuous assay were applied with the system to analyse freshwater and marine samples.
In this article, we report the development of a continuous assay with potential for high throughput using fluorescence resonance energy transfer-based peptide substrates.
Each is a continuous assay, free from interferences caused by other components present in crude enzyme preparations, and allows meaningful and reproducible kinetic parameters to be determined.
The continuous assay, using glucokinase (Glk) and glucose-6-phosphate dehydrogenase (Gpd) from Thermotoga maritima, demonstrated robust activity over a range of temperatures (75 90 °C) and pH values (6.8– 8.5).
Similar(21)
However, there are no continuous assays that provide C-terminal substrate sequence specificity for inflammatory caspases.
This fluorogenic peptide is a very sensitive substrate for continuous assays and for the in situ determination of matrix metalloproteinase activity.
MMP activity was assessed by Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 synthetic fluorogenic substrate (Bachem, Weil am Rhein, Germany) in continuous assays [ 34].
B) The existing tracker was optimized for animals on an agar plate, where prolonged continuous assays often fail (e.g., due to the animal escaping or burrowing).
The K i values of some of the mutants with cathepsin L were further confirmed by the Dixon method in continuous assays using [ S] ≪ KM.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com