The continuous amplification program consisted of one cycle at 95°C for 15 min, followed by 40 cycles at 94°C for 20 s, 60°C (Staphylococcus, Enterobacteriaceae, Pseudomonas, Bacillus cereus, S. agalactiae), 55°C (Enterococcus, Clostridium coccoides group, Clostridium leptum, Atopobium cluster, Prevotella, Bifidobacterium ) or 50°C (Bacteroides fragilis group) for 20 s and 72°C for 50 s.
Given that LAMP and many other continuous amplification protocols frequently produce spurious products because of non-specific and often template-independent amplification, CHA circuitry has true utility in redeeming these reactions for diagnostic applications.
Continuous PCR amplification of KIF7 exons from whole blood mRNA was not successful.
Intensely autobiographical, it seems a continuation and amplification of "Extravagaria".
Ultra-high Brillouin gain of up to 50 dB amplification of a small continuous wave (CW) seed for a moderate CW pump power of 300 mW was achieved.
At the end of each run, melting curve profiles were produced by cooling the sample to 65°C for 15 s and then heating slowly at 0.20°C⋅s−1 up to 95°C with continuous measurement of fluorescence to confirm amplification of specific transcripts.
Melting curves were generated after each run (cooling the sample to 65°C for 15 s and then heating slowly at 0.2°C/s up to 95°C with continuous measurement of fluorescence) to confirm amplification of specific transcripts.
The data acquisition and control system included amplification of the hydrophone signals, bandpass filtering, digitization, and continuous recording on a high performance digital recorder for offline processing of the time series.
Specifically, we use a biofabricated catechol-modified chitosan redox cycling system to provide a significant amplification of the generated oxidizing current of clozapine through a continuous cycle of clozapine reduction followed by re-oxidation.
To evaluate the amplification of a specific sample, final melting curve (from 60°C up to 95°C) was added under continuous fluorescence measurements.
