In replicates in which a fertility type successfully colonized the hybrid habitat, we continued the simulation to monitor its persistence.
There might not be enough computing power to continue the simulation if billions of inhabitants of a virtual world started creating their own virtual worlds with billions of inhabitants apiece.
We continued the simulation until the whole model was filled up with proppant.
Then the end state information is stored and next branch information is loaded to continue the simulation.
To test for such stable and distinct states we continued the simulation for 20 generation times, without further replication, and examined the Lys/Lyt ratios (see Methods).
Given the first generation, we continue the simulation using a birth/death process, for a number of generations (birth-and-death cycles).
If needed, we continued the simulation again with a second change of primary and/or polarizing signals.
The ability of the VENSIM DLL to pause and continue the simulation at predefined points in time enabled an automation of this otherwise quite cumbersome task.
The clock advances of the simulated "time to remission" and a test is performed to check if there is time left to continue the simulation (according to the fixed time horizon) or not.
We continue the simulations until a genotype goes extinct or 100,000 rounds have passed.
