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Sure enough, on Tuesday night, the symphony of dits and dahs continued for hours at the receiving station, as amateur Morse operators from around the world chimed in to say hello.
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Sporadic clashes continued for hours.
This continued for hours.
Hoping to do reconnaissance for the upcoming route, he continued walking for hours, at one point climbing onto a ledge and looking south.
After an additional 4 hours, 1 volume of complete medium containing 10% FCS (undialyzed) was added and incubation was continued for 16 hours at 37°C.
Stirring is continued for four hours at 40°C in order to ensure the maturation of the microstructure walls. 5.
Heavy fire from Ottoman rifles and machine guns continued for several hours at Katia, and the Ottomans gradually pressed in on the yeomanry's front and flanks.
Incubation was continued for 4 hours at either 30°C or 38°C prior to flow cytometric analysis [26].
The reaction was commenced by adding 100 μl of 2% beechwood xylan (Sigma-Aldrich, Missouri, United States), suspended in 50 mM citrate buffer, pH 6.0, and the reaction was continued for 2 hours at 37°C.
Incubation was continued for 12 hours at 37°C and in 5% CO2, after which the cells were harvested and processed with the QIAamp RNA Blood Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions, to isolate RNA.
Expression of the transaminase gene VAMT was induced by addition of isopropyl-β-D-thiogalactoside (IPTG) to a final concentration of 1 mM when the OD620 of the culture reached 0.6 to 0.8 and incubation was then continued for 24 hours at 15°C and 180 rpm.
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CEO of Professional Science Editing for Scientists @ prosciediting.com