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Continued passage led to mutations in the non-structural genes although these mutations did not significantly enhance replication of the original replicon.
Early work showed that most influenza virus strains isolated from humans are predominantly filamentous and, upon continued passage in egg or tissue culture, adopt a more spherical morphology, which correlated with increased virus titers.
After continued passage, small foci of rounded cells developed, and rapid and progressive cell death throughout the flask ensued in a pattern uncharacteristic of the viruses commonly isolated from dogs.
For the first one to three passages, cells grown in USSC growth mediumACF proliferate and maintain their morphology, but with continued passaging the cells form spherical cell aggregates.
After continued passaging and culturing, the expression of CD34, Flk-1 and Abcg-2 mRNA was still maintained at a high-rate compared with NSP cells.
Consistent with a model of constant recombination between homologous chromosome ends, most new mutations became homozygous following continued passaging (data not shown).
With continued passaging, the rate of cell proliferation gradually decreased around day 60 in cultures grown in standard medium (containing 30 mg L−1 methionine), whereas cells cultivated under MetR were still actively proliferating until day 120; from the onset of treatment, control cells completed 21 cumulative population doublings (cPDL), whereas MetR cells completed 34 cPDL (Fig. 1A).
Ben Edgerton Like Channing Tatumn Like Channing Tatum before him, Edgerton is set to continue his passage from roughly sanded beefcake to smoothy rounded actor.
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