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The center-of-mass (CoM) of the response distribution is projected onto the cortex to determine the likely target site, implicitly assuming the existence of a single, contiguous target.
To date, lhRNAs capable of producing more than two independent siRNAs have only been used against contiguous target sequences.
To date, lhRNAs expressed from RNA Pol III promoters have been used against a single contiguous target sequence in mammalian cells.
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The results indicated that an MDC-Analyzer-facilitated combinatorial strategy represents an efficient tool for the directed evolution of functional enzymes with multiple contiguous targeting sites.
One very recent work [9] has considered use of matching pursuit to find a sparse, non-contiguous target impulse response (TIR), and it is shown to yield a lower mean squared error (MSE) compared to the conventional contiguous approach.
Since ∼60 bp hairpin RNAs should be capable of providing a substrate for at least three catalytic reactions involving Dicer, we have examined the possibility of introducing three distinct non-contiguous target sequences that, if processed by Dicer, are capable of generating highly-effective independent siRNA species.
In [9], effective sidelobe suppression algorithms were introduced for discrete point targets and contiguous scattering targets, which we refer to as the CLEAN algorithm in this paper.
In this study, we apply an iterative sidelobe removal algorithm based on the CLEAN algorithm [9], which was proven to remove sidelobe components effectively in a binary-coded pulse compression radar system dealing with contiguous scattering targets.
Rats were irradiated with a 200 Gy entrance dose using two different MB arrays which generated two contiguous radiation targets (4.1 and 2.7 mm long, 1.5 mm high).
(B) Contiguous deployment of weather targets leads to an overlap between autocorrelation sidelobes of adjacent targets, which vastly distorts the MF output even with the code of low autocorrelation sidelobe.
In New Jersey, the goal is to target contiguous properties to restore floodplains.
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