For STB-A, a fully finished contiguous sequence of 4,482,059 bp was obtained using ∼80,000 shotgun Sanger reads, Illumina-generated reads and finishing12,28.
Remaining gaps in the genome sequence were closed by PCR walking [48] between un-linked contiguous sequences to finish the genome sequence.
Gene order (synteny) was poorly conserved between Xiphinematobacter and the five other Verrucomicrobial species with finished (contiguous) genomes (supplementary fig. S5, Supplementary Material online).
Sequencing and finishing of three contiguous H. melpomene BACs across this region was carried out by the Wellcome Trust Sanger Institute (accession numbers: CR974474, CT955980, CU367882).
To assemble this highly repetitive genome, we combined reads that were generated from four next-generation sequencing platforms by using hybrid assembly approaches to finish two-chromosome contiguous sequences without gaps by validating the data with optical restriction maps and Sanger sequencing.
This represents one of the first de novo genomes finished into a single contiguous sequence using RS II data alone (that is, without addition of other next-generation sequence data or manual finishing steps).
Clustering of the shotgun reads (Table 1, Additional file 1) and manual finishing of the assembly resulted in 820 contiguous sequences (contigs) with an N50 of 71,261 bp and an average genome coverage of 47×.
While the suspects were found in Watertown, several miles from the site of the marathon's finish line in Boston, the two cities are contiguous at points; Watertown is not "about seven miles west of Boston".
Then comes a coastal and island section around Puget Sound before the final mountains of the Olympic range and a finish along the spectacular Pacific coast to Cape Alava, the westernmost point in the contiguous 48 states.
To finish the genome sequence, remaining gaps were closed by PCR walking between un-linked contiguous sequences [21].
"Baltimore County and Baltimore City are contiguous.
